Online Library. Theme: Biology
Found: 72
Found: 72
| Title: | A development of a PCR-RFLP test system for the identification of mitochondrial lines of the Pelophylax ridibundus lake frog in Kazakhstan |
| Author: | Ермаков Олег Александрович Иванов Александр Юрьевич |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | Background. Molecular typing by PCR-RFLP method allows to identify the spe-cific attribution of an organism that has a weak phenotypic difference. The main advantage of this research method is the capacity to analyze a large number of samples without apply-ing the sequencing method. The purpose of the work is to develop a test-system for the identification the matrilines of marsh frogs of the Pelophylax ridibundus complex from Kazakhstan. Materials and methods. The analysis was based on the variability of the prima-ry structure of the mitochondrial gene the second subunit of dehydrogenase (ND2) which is a species-specific marker. Subsequently a search for marker nucleotide substitutions speci-fic for each lineage and recognition sites for the HaeIII and TasI restriction endonucleases was conducted. Results. As a result of the research, it was confirmed that on the territory of Kazakhstan inhabiting three main forms of lake frogs where two of them are native (Balkhash, Syrdarya), and invasive Anatolian form - P. cf. bedriagae. Conclusions. The authorsʼ PCR-RLFP test technique is a straightforward and reliable tool for detecting mitochondrial lineages in the Pelophylax ridibundus complex marsh frogs and may be used successfully in mass screening investigations. |
| Publication date: | 2022 |
| Publishing house: | Пензенский государственный университет (Пенза) |
| Title: | A mollusk Planorbarius corneus is an intermediate host of the infectious agent of rostand’s “anomaly p” in green frogs |
| Author: | Ермаков Олег Александрович Иванов Александр Юрьевич |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | Here we present the first new data about the mysterious "anomaly P" of green frogs (genus Pelophylax) in about 50 years. We established that the gastropod Planorbarius corneus could be an intermediate host (or vector) of the infectious agent of the anomaly P. Symmetrical cases of polydactyly, the anomaly "cross" and heavy cases of the anomaly P, which were previously found in natural populations in the European part of Russia and recently obtained in laboratory, can be caused by this infectious agent. As the most probable cause, we assume a species of trematodes, for which the first intermediate host is P. corneus, from which they infest tadpoles of green frogs. |
| Publication date: | 2019 |
| Publishing house: | Folium Publishing Company |
| Title: | A new record of Bufo gargarizans complex (Bufonidae, Anura) from Truong Son Mounts, Ha Tinh and Ha Giang Provinces, Vietnam based on molecular evidence with a description of a new species |
| Author: | Ермаков Олег Александрович |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | Based on a combination of molecular and morphological data, we herein report a new species within the bufonid Bufo gargarizans species complex. This is a widespread species complex with distribution from eastern Russia and the Korean Peninsula to China and the Ryukyu Islands of Japan. Records of this species have been documented in the Guangxi and Yunnan Provinces near the border with Vietnam and, for the first time from Vietnam, in Ha Giang Province. The new record of Bufo cf. gargarizans from Vietnam is from Ha Tinh Province. This species has never been reported from Vietnam so far south, about 550 km south from the previously known locality in Ha Giang Province. The female specimen was found in the Ha Tinh Province, Vu Quang National Park of central Vietnam and two specimens (male and female) were found Ha Giang Province. They are clearly distinguished from B. gargarizans and all the mentioned species by a specific color pattern on the belly and creamy-yellowish throat with large, bright red speckles. Genetic divergences of three Vietnam specimens from Ha Giang and Ha Tinh Provinces in the ND2 gene sequences between the B. sp. nov. and all other congeners ranged from 4.3% (with B. andrewsi) to 7.0% (with B. stejnegeri). We give a description of the morphological characters and coloration of the new record and provide an expanded diagnosis. |
| Publication date: | 2024 |
| Publishing house: | Multidisciplinary Digital Publishing Institute (MDPI) |
| Title: | A new record of odd-scaled snake (Serpentes, xenodermidae) from vietnam: Expanded description of parafimbrios vietnamensis based on integrative taxonomy |
| Author: | Ермаков Олег Александрович |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | Based on the combination of molecular and morphological data, we herein report the second known finding of the xenodermid snake species Parafimbrios vietnamensis Ziegler, Ngo, Pham, Nguyen, Le & Nguyen, 2018. The male individual was found in the Yen Bai Province of northwestern Vietnam, more than 200 km from the type locality in Lai Chau Province. Genetic divergence between the newly-collected male and the holotype was low (1.7%), and is in agreement with morphological data that supports that they are conspecific. We give a detailed description of the morphological characters and coloration of the new record and provide an expanded diagnosis of P. vietnamensis. Parafimbrios is a poorly-understood genus, and our recent discovery brings the total number of known specimens of the genus to nine, 1/3 of them having been found in Vietnam (one specimen of P. lao and now two specimens of P. vietnamensis). |
| Publication date: | 2021 |
| Publishing house: | PENSOFT PUBLISHERS |
| Title: | A new species of acanthosaura gray, 1831 (Squamata: Agamidae) from central highlands, Vietnam |
| Author: | Ермаков Олег Александрович |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | Herein, we describe a new agamid species of the genus Acanthosaura from Central Highlands of Vietnam: Gia Lai Province, Kon Chu Rang NR, and Kon Tum Province: Kon Plong and Ngoc Linh Mountain based on morphological and molecular data. Acanthosaura prasina sp. nov. is distinguished from all congeners by a combination of the following morphological characters. Acanthosaura prasina sp. nov. differs from A. capra, A. murphyi, and A. nataliae by the presence of a short spine on each side of the neck and a smaller body size. From the known species of "lepidogaster" complex and A. coronata it differs in the arrangement of dorsal crests with a distinct double row of vertebral scales, from A. brachypoda in a smaller body size, longer tail and limbs, fewer supra- and infralabials and coloration patterns with a white area on lips and shoulders; from A. lepidogaster by lacking a dark marking on the nape and the absence of a distinct diastema; from A. phongdienensis by lacking a dark marking on the nape. We provide a cyt b-based estimation of diversity of the genus Acanthosaura. A. prasina sp. nov. differs from A. phongdiensis, A. coronata, A. lepidogaster, A. armata, A. crucigera, A. nataliae, A. phuketensis, and Acanthosaura sp. SK in p-distances of 15.4, 24.5, 14.7, 14.6, 16.3, 14.6, 15.4, and 24.4%, respectively. The new species currently known from three localities from Central Highlands was recorded from 800 to 1700 m a.s.l. in the evergreen polydominant forests in the mountainous regions of Gia Lai and Kon Tum provinces. This is the 16th species of Acanthosaura and the ninth species of the genus from Vietnam. |
| Publication date: | 2020 |
| Publishing house: | Folium Publishing Company |
| Title: | A record of alien pelophylax species and widespread mitochondrial dna transfer in kaliningradskaya oblast’ (The baltic coast, russia) |
| Author: | Ермаков Олег Александрович Иванов Александр Юрьевич |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | Alien species can strongly impact local environments and compete against native species, which can lead to their extinction. Marsh frogs of the Pelophylax ridibundus complex are one of the most invasive amphibians in Northern Eurasia. It was previously thought that three water frog species of the genus Pelophylax (the marsh frog, P. ridibundus, the pool frog, P. lessonae and their hemiclonal hybrid, the edible frog, P. esculentus) inhabited Kaliningradskaya Oblast' along the Russian Baltic coast. However, based on our study of the intron-1 of the nuclear serum albumin gene, two other marsh frog species were detected (the Balkan marsh frog, P. kurtmuelleri, and the Anatolian marsh frog, P. cf. bedriagae) as well as putative hybrids between P. ridibundus and P. cf. bedriagae. The majority of individuals of P. ridibundus and hybrids between P. ridibundus and P. cf. bedriagae had mitochondrial (mt) DNA of P. lessonae, while all others featured the P. kurtmuelleri mtDNA. The prevalence of P. lessonae mtDNA haplotypes in populations of P. ridibundus from the Baltic Coast of Russia suggests that local individuals of the latter species originated from crosses between P. esculentus individuals. Two hypotheses could explain the records of P. kurtmuelleri and P. cf. bedriagae in the region. The establishment of local populations of the first species could have occurred via postglacial dispersal from the Balkan refugium. The origin of local P. cf. bedriagae could be an occasional introduction of individuals from the Ponto-Caspian region. Since our study is preliminary (19 individuals), in the future it would be important to continue the study of water frogs in Kaliningradskaya Oblast' and neighboring countries by applying multiple genetic markers. Additional genetic markers will enable researchers to study routes of dispersal and introductions of marsh frogs, to clarify peculiarities of their hybridization and distribution, and to evaluate the impact of P. kurtmuelleri and P. cf. bedriagae on the reproduction success of hybridogenous populations and abundance of local amphibians. |
| Publication date: | 2020 |
| Publishing house: | Regional Euro-Asian Biological Invasions Centre |
| Title: | A review and molecular study of bugs of the genus Cimex in Russia with an emphasis on the findings on bats. |
| Author: | Смирнов Дмитрий Григорьевич |
| Type: | Article |
| Kind: | Electronic copy |
| Publication date: | 2024 |
| Publishing house: | Department of Agricultural and Food Sciences |
| Title: | Age Structure of Water Frogs of the Genus Pelophylax in the Middle Volga River Region (European Russia) |
| Author: | Ермаков Олег Александрович |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | An analysis of the age structure of three water frog species (P. lessonae, P. ridibundus, P. esculentus) in the Middle Volga region of Russia was conducted. The skeletochronological method determined their maximum lifespans to be 7, 6, and 4 years, respectively. This study revealed specific characteristics of the age structure of Pelophylax frogs in relation to their taxonomic composition when cohabiting (population system type) and the degree of habitat transformation. Differences in body size and lifespan were observed among cryptic forms exhibiting mitochondrial DNA replacement and the presence of various types of heterozygous and homozygous alleles. Water frogs of the genus Pelophylax have a complex taxonomic structure, including hybrid species and morphologically cryptic forms, as well as massive mitochondrial DNA introgression. The aim of our paper was to analyze the age structure of three species of water frogs (P. lessonae, P. ridibundus, and P. esculentus) in the Middle Volga River region (European Russia). Based on the skeletochronological method, we determined their maximum lifespans of seven, six, and four years, respectively. The onset of sexual maturity in the marsh frogs occurred after two and four winterings in males and females (respectively), in the pond frog—one to two years, and in the edible frog—one to three years. This study revealed specific characteristics of the age structure of Pelophylax frogs in relation to their taxonomic composition when cohabiting (population system type) and the degree of habitat transformation. Differences in body size and lifespan were observed among cryptic forms exhibiting mitochondrial DNA replacement and the presence of various types of heterozygous and homozygous alleles. |
| Publication date: | 2025 |
| Publishing house: | Multidisciplinary Digital Publishing Institute (MDPI) |
| Title: | Assessment of species specificity of the mitochondrial genome of russet souslik Spermophilus major Pallas, 1778 |
| Author: | Ермаков Олег Александрович Титов Сергей Витальевич |
| Type: | Article |
| Kind: | Electronic copy |
| Publication date: | 2018 |
| Publishing house: | Московский педагогический государственный университет |
| Title: | Biodiversity of Marine Interstitial Ciliates in the Intertidal Zone of the White Sea: A Dataset from the Chernaya River Estuary, Kandalaksha Gulf. |
| Author: | Esaulov Anton |
| Type: | Article |
| Kind: | Electronic copy |
| Publication date: | 2023 |
| Publishing house: | MDPI |
| Title: | Biodiversity of Terrestrial Testate Amoebae in Western Siberia Lowland Peatlands |
| Author: | Чернышов Виктор Александрович Esaulov Anton |
| Type: | Article |
| Kind: | Electronic copy |
| Publication date: | 2023 |
| Publishing house: | Multidisciplinary Digital Publishing Institute (MDPI) |
| Title: | Community assembly and co-occurrence network complexity of interstitial microbial communities in the Arctic (investigation of ciliates in the White Sea intertidal zone) |
| Author: | Esaulov Anton |
| Type: | Article |
| Kind: | Electronic copy |
| Publication date: | 2023 |
| Publishing house: | Elsevier |
| Title: | Contact Zone of European (Meles meles Linnaeus, 1758) and Asian (M. leucurus Hodgson, 1847) Badgers (Mustelidae, Mammalia) in the Right- and Left-Bank Districts of the Saratov Region |
| Author: | Титов Сергей Витальевич |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | The DNA studies of badgers (Meles sp.) from the right-bank and left-bank areas of the Volga River in the Saratov region are described. Asian badgers (Meles leucurus Hodgson, 1847) inhabit the Left Bank of the Volga River in the Saratov region, while European badgers (M. meles Linnaeus, 1758) inhabit the Volga Right-Bank districts of the region; however, Asian badgers have been found in Khvalynsky district of the Saratov Region in addition to the European badger. Despite the sufficient number of publications devoted to the Asiatic badger distribution in the Vyatka–Kama region and the Volga region and the studies devoted to the development of systematics of the genus Meles in Russia, the question of the boundaries of the ranges of European and Asiatic badgers and the zones of their sympatry (parapatry) in the Volga–Kama region has not been fully investigated to date. Our work is devoted to the study of this question. The analysis of biological material that we have collected has shown that all five of the studied badger individuals from the Saratov Volga region phenotypically look like Asian badgers; however, DNA analysis showed that only two of them were M. leucurus and other three were heterozygous individuals carrying genes from both species and were identified as hybrids. The remaining 29 individuals were captured in the right-bank areas of the region. Samples were taken from badger individuals from the northern to southern borders of the region in the areas located along the Volga River on the Volga Upland and in the Oka–Don Plain. Among them, one individual from Khvalynsky district turned out to be an Asian badger, one individual from Krasnoarmeysky district was a hybrid of these two species, and three individuals, one from Tatishchevsky, the second from Volsky and the third from Khvalynsky districts showed introgression of Asian badger genes into the genotype of European badger. Thus, we have managed to find out that, at this stage of the development of the climatic cycle phase in the Lower Volga Region characterized by warming winters, the Volga River, with its two reservoirs within the Saratov region, is not an absolute zoogeographical boundary and badgers whose species are characterized by winter sleep can under certain conditions overcome, most likely on ice, both the river itself and the lake parts of the Volgograd and Saratov reservoirs. According to the revealed introgression in some individuals from different areas on the Volga Upland of the right bank of the Saratov region, it can be assumed that such traveling across the Volga River took place earlier, possibly before its flow was regulated by dams. |
| Publication date: | 2024 |
| Publishing house: | Springer |
| Title: | Continent-Wide Distribution of CMTV-Like Ranavirus, from the Urals to the Atlantic Ocean |
| Author: | Ермаков Олег Александрович Zaks Svetlana |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | Ranavirosis is an emerging infectious disease of amphibians, fish, and reptiles caused by large dsDNA viruses of the genus Ranavirus associated with morbidity and mass mortalities worldwide. They are considered to be one of the major drivers of the ongoing amphibian biodiversity crisis. In this study, we investigated the prevalence and genetic diversity of ranaviruses in native and invasive populations of water frogs (Pelophylax spp.) across Russia using the DNA sample collection established in 2006–2016. The collection included samples collected in the wild and samples from wild-caught water frogs that had been kept in laboratories for a period of time. Overall, 52 out of 590 (8.8%) of wild frogs from 18 out of 94 (19.1%) sampling sites tested positive, including samples from invasive populations. Among the captive frogs, 71 out of 263 (27.0%) were positive and they had a significantly higher relative viral load. We found six major capsid protein gene haplotypes from 22 positive samples, all belonging to the common midwife toad virus (CMTV-like) ranaviruses, at multiple sites within the basins of three of Europe's largest rivers (Volga, Dnieper, and Don). Combined with previously published data, this study provides evidence for a continent-wide distribution of CMTV-like ranaviruses in Europe and strengthens the hypothesis of their endemism on the continent. Our study also highlights that the water frogs are important hosts for ranaviruses and could potentially act as vectors for infection transmission. |
| Publication date: | 2025 |
| Publishing house: | Springer Verlag |
| Title: | Creating an integrated information system for the analysis of mammalian fauna in the Russian Federation and the preliminary results of this information system |
| Author: | Ермаков Олег Александрович Смирнов Дмитрий Григорьевич Титов Сергей Витальевич |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | The current capacity for the purposeful study of the mammalian fauna of Russia using public databases is discussed. A list of species of Russian fauna compiled under united principles is lacking and the lists of species published in different departments are inconsistent. Systems that can monitor changes in the qualitative or quantitative composition of the fauna are not yet sufficiently refined. The systematization of previously accumulated faunal data requires the involvement of qualified taxonomists. Nevertheless, initial work on the “Mammals of Russia” database, which currently contains 16 512 records on 235 species of mammals, has allowed us to positively assess the prospects for further ordering of faunal information within the country. |
| Publication date: | 2018 |
| Publishing house: | Товарищество научных изданий КМК |
| Title: | Detection of glacial refugia and post-glacial colonization routes of morphologically cryptic marsh frog species (Anura: Ranidae: Pelophylax) using environmental niche modeling |
| Author: | Ермаков Олег Александрович Иванов Александр Юрьевич |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | Studying the distribution of morphologically cryptic animal species is always a very difficult task. Because most marsh frog species (the Pelophylax ridibundus complex) are cryptic, we used molecular markers to identify them. Three marsh frog species (P. ridibundus, P. kurtmuelleri and P. cf. bedriagae) inhabit the northern part of Western Palearctic. We created a database of localities and built models of their modern distribution. These models showed that the most suitable habitats are on the north of the Mediterranean region for P. cf. bedriagae, temperate Europe for P. ridibundus, and the Balkan coastal areas for P. kurtmuelleri. The projection of the modern ecological niches under the late-Quaternary climatic conditions showed that the range of P. kurtmuelleri remained largely unchanged during the period, whereas the ranges of P. cf. bedriagae and especially P. ridibundus changed greatly over time. During the Last Glacial Maximum, the presumed range of P. cf. bedriagae covered a relatively large area in the north of the Mediterranean region and the south of European Russia. Glacial refugia of P. ridibundus were apparently located in the northern Balkans, the northern coast of the Black and Azov seas, and possibly in Western Europe. The northward long-distance post-glacial dispersal of P. ridibundus occurred from refugia in the northeastern Balkans and the Black-Azov seas region. Since the Late Pleistocene, suitable habitats for P. cf. bedriagae in southern Russia began to decline, but local habitats for P. ridibundus become more suitable. Therefore, a mosaic of populations consisting of these both species and their hybrids has now been found here. |
| Publication date: | 2024 |
| Publishing house: | Multidisciplinary Digital Publishing Institute (MDPI) |
| Title: | Distribution and origin of two forms of the marsh frog Pelophylax ridibundus complex (anura, ranidae) from kamchatka, based on mitochondrial and nuclear DNA data |
| Author: | Ермаков Олег Александрович Титов Сергей Витальевич |
| Type: | Article |
| Kind: | Electronic copy |
| Publication date: | 2019 |
| Publishing house: | Pleiades Publishing, USA |
| Title: | Distribution of activities of alkaline carboxypeptidases in tissues of laboratory animals of different species |
| Author: | Schetinina Natalia Saldaev Damir Вернигора Александр Николаевич |
| Type: | Article |
| Kind: | Electronic copy |
| Publication date: | 2002 |
| Publishing house: | Pleiades Publishing, Ltd. (Плеадес Паблишинг, Лтд) (Род-Таун) |
| Title: | Distribution of benthic testate amoeba assemblages along a water depth gradient in freshwater lakes of the Meshchera Lowlands, Russia, and utility of the microfossils for inferring past lake water level |
| Author: | Malysheva Elena Цыганов Андрей Николаевич |
| Type: | Article |
| Kind: | Electronic copy |
| Annotation: | Testate amoebae are important components of benthic communities in freshwater lakes, where they play an essential role in decomposer food webs. They are used widely in paleoecological investigations because of their high taxonomic diversity, well-defined ecological preferences and decay-resistant tests. Studies of testate amoeba assemblages in lake surface sediments are necessary to better understand lake ecosystem function and improve the use of these organisms as bio-indicators in paleoecology. This study explored the use of testate amoebae as proxies for inferring past water level in freshwater lakes, and expanded upon the limited body of research into lake testate amoebae in Russia. Our results indicate that species composition of testate amoeba assemblages in the lakes was typical for such biotopes, with most of the species belonging to the genera Difflugia, Centropyxis, Arcella and Euglypha. Analysis of variation of testate amoebae along a water-depth gradient showed that three assemblage types could be distinguished: shallow-water (0–4.5 m), intermediate-water-depth (4.5–20.5) and deep-water (20.5–33 m). Deep-water assemblages did not contain any unique taxa and were dominated by eurybiotic and planktonic species. Species diversity was highest in the intermediate-water-depth assemblages and lowest in deep-water ones. Although variations in testate amoeba assemblages across water depth in freshwater lakes are complex and context-dependent, there are clear patterns in species composition and diversity, which can be used to infer past lake water levels. Future studies on the effect of water depth on testate amoeba assemblages in diverse types of freshwater lakes should increase the utility of the method. |
| Publication date: | 2019 |
| Publishing house: | Springer |
| Title: | Distribution of soil testate amoeba assemblages along an elevation gradient on Mount Fuji (Japan) |
| Author: | Чернышов Виктор Александрович |
| Type: | Article |
| Kind: | Electronic copy |
| Publication date: | 2022 |
| Publishing house: | ELSEVIER |